Comparative analysis of collagen-1 amongst locally-farmed tilapia samples as potential xenograft for partial-thickness burns


  • Pinyapat Siriruck Nawamintharachinuthit Triamudomsuksanomklao School, Bangkok, Thailand
  • Woraphat Pongpoon Nawamintharachinuthit Triamudomsuksanomklao School, Bangkok, Thailand



Collagen type-I, Xenograft, Burn treatment


Background: Tilapia farming is amongst the most lucrative in terms of yield and return of investments in the aquaculture industry in Thailand. The sustainable supply of tilapia (Oreochromis spp.) can be exploited to reconsider the fish species as a huge potential for Thailand’s medical sector, particularly on xenograft as an innovative treatment for burns.

Methods: Collagen content quantification by high-performance liquid chromatography (HPLC) method is done on two samples: Chitralada I tilapia (Thai locally cultured strain of Oreochromis niloticus) and pink tilapia (commercially cultured strain by Charoen Pokphand Foods PCL) skin to compare the amount of type I collagen (COL-I) by measuring one of its major amino acids, hydroxyproline, with Nile tilapia skin which has been used as xenograft in Brazil.  

Results: The results showed that there is no significant difference in the amount of hydroxyproline amongst the two strains. Chitralada I tilapia sample contains 9.01 g/100g (9.01%) and pink tilapia sample contains 18.55 g/100g (18.55%) of hydroxyproline respectively.

Conclusions: Since there are minor differences between the amount of hydroxyproline in Thai locally-farmed tilapia and Nile tilapia; therefore, Thai locally-farmed tilapia xenograft can be a possible choice for burn treatment.


Metrics Loading ...


Froese R, Walters C, Pauly D, Winker H, Weyl OL, Demirel N, et al. A critique of the balanced harvesting approach to fishing. ICES J Marine Sci. 2015;73:1640-50.

Lee TC, Mohd Pu'ad NAS, Alipal J, Muhamad MS, Basri H, Idris MI, et al. Tilapia wastes to valuable materials: A brief review of biomedical, wastewater treatment, and biofuel applications. Materials Today: Proceedings. 2022;57:1389-95.

Whitaker P, Garcia P. Reuters: Brazilian doctors use fish skin to treat burn victims, 2017. Available at: Accessed on 21 January 2023

Hutson PR, Crawford ME, Sorkness RL. Liquid chromatographic determination of hydroxyproline in tissue samples. J Chromatography B. 2003;791:427-30.

Hu Z, Yang P, Zhou C, Li S, Hong P. Marine collagen peptides from the skin of Nile tilapia (Oreochromis niloticus): Characterization and Wound Healing Evaluation. Marine Drugs. 2017;15:102.

Cen L, Liu W, Cui L, Zhang W, Cao Y. Collagen Tissue Engineering: Development of novel biomaterials and applications. Pediatric Res. 2008;63:492-6.

Gómez-Guillén MC, Giménez B, López-Caballero ME, Montero MP. Functional and bioactive properties of collagen and gelatin from alternative sources: A Review. Food Hydrocolloids. 2011;25:1813-27.

Szpak P. Fish Bone Chemistry and ultrastructure: Implications for taphonomy and stable isotope analysis. Journal of Archaeological Science. 2011;38:3358-72.

Neuman RE, Logan MA. The determination of Hydroxyproline. Journal of Biological Chemistry. 1950;184:299-306.

Shpichka A, Butnaru D, Bezrukov EA, Sukhanov RB, Atala A, Burdukovskii V, et al. Skin tissue regeneration for burn injury. Stem Cell Res Therapy. 2019;10.

Yamamoto K, Igawa K, Sugimoto K, Yoshizawa Y, Yanagiguchi K, Ikeda T, et al. Biological safety of fish (tilapia) collagen. BioMed Res Int. 2014;2014:1-9.

Matsumoto R, Uemura T, Xu Z, Yamaguchi I, Ikoma T, Tanaka J. Rapid oriented fibril formation of fish scale collagen facilitates early osteoblastic differentiation of human mesenchymal stem cells. J Biomed Materials Res Part A. 2014;103:2531-9.

Alves AP, Lima Júnior EM, Piccolo NS, de Miranda MJ, Lima Verde ME, Ferreira Júnior AE, et al. Study of tensiometric properties, microbiological and collagen content in Nile tilapia skin submitted to different sterilization methods. Cell Tissue Banking. 2018;19:373-82.

Lima Junior EM, Picollo NS, Miranda MJB, Ribeiro WLC, Alves APNN, Ferreira GE, et al. Uso da pele de tilápia (Oreochromis niloticus), como curativo biológico oclusivo, no tratamento de queimaduras. Rev Bras Queimaduras 2017;16:10-7.

Chen J, Gao K, Liu S, Wang S, Elango J, Bao B, et al. Fish collagen surgical compress repairing characteristics on wound healing process in vivo. Marine Drugs. 2019;17:33.






Original Research Articles