Effect of vitamin E on biochemical and ultrastructural changes in acrylamide-induced renal toxicity in rats
Keywords:Acrylamide, Kidney, Vitamin E, NF-kβp65, Bcl-2, Ultarstructure
Background: Acrylamide (ACR) is a widely used chemical in industry and it accounts for major health problems as it has been detected in highly consumed food items, carbohydrate-rich food items cooked at high temperature. Accordingly the population is highly exposed to ACR. The aim of the study was to assess the effect of vitamin E on biochemical and ultrastructure changes in acrylamide-induced renal toxicity in wistar albino rats.
Methods: Three groups of adult albino wistar rats weighing about (200-250 gm) were used in this study to investigate the effect of vitamin E on acrylamide induced renal toxicity; 10 rats in each group; Group I: Control group, Group II: Acrylamide treated and Group III: Acrylamide-Vit E treated group. Blood samples were collected for estimation of serum creatinine, blood urea nitrogen (BUN), lactic dehydrogenase (LDH) and albumin. Kidneys specimens were processed for light and electron microscopic studies. Kidney sections were stained with H&E, MT, PAS and immuno-histochemical stains for detection of NF-kβp65 and Bcl-2. Morphometric study was done followed by biochemical and morphometric statistical analysiss.
Results: Acrylamide treated rats showed degeneration of cells lining PCT and DCT, atrophy of glomeruli and fibrosis. Ultrastructurally; tubules lining cells showed loss of microvilli, basal membrane in foldings and mitochondrial changes. Podocytic changes include: Vacuolation, irregularity and disorientation of their processes and thinning of glomerular basement membrane. Significant increase in the mean number of NF-kβp65 positive cells and decrease in the mean area% of Bcl-2 immuno-expression, increase in the mean area % of collagen fibers and urinary space diameter and decrease in thickness of epithelial cells of PCT and DCT were also observed. Serum creatinine, BUN anLDH were increased whereas serum albumin was decreased. Vitamin E co-administration with ACR improve all these histological, ultrastructural and biochemical changes.Conclusions: Acrylamide induced renal toxicity could be ameliorated by vitamin E co-administration.
Mannaa, F, Abdel-Wahhab MA, Ahmed HH, Park MH. Protective role of Panax ginseng extract standardized with ginsenoside Rg3 against acrylamide-induced neurotoxicity in rats. J Appl Toxicol. 2006;26:198-206.
Grivas S, Jagerstad M, Lingnert H, Skog K, Törnqvist M, Åman P. Acrylamide in food, mechanisms of formation and influencing factors during heating of foods. Sweden: Swedish National Food Administration. 2002;46(4):159-72.
Schultzova K, Tekel J. Acrylamide monomer occurrence in sugar. Deutsche Lebensmittel Rundschau. 1996;92(9):281-2.
Friedman M. Chemistry, biochemistry, and safety of acrylamide- A review. J Agricult Food Chem. 2003;51:4504-26.
Exon JH. A review of the toxicology of acrylamide. J Toxicol Environ Health B Crit Rev. 2006;9:397-412.
Tareke E, Rydberg P. Analysis of Grape seeds. 2005;(17):4998-5006.
Surdyk, N, Rosen J, Andersson R, Aman P. Effects of asparagine, fructose and baking conditions on acrylamide content in yeast-leavened wheat bread. J Agric Food Chem. 2004;52:2047-51.
Pruser KN, Flynn NE. Acrylamide in health and disease. Front Biosci. (Scholar edition). 2011;3:41-51
Guyton AC, John E. Medical Physiology. 11th Edition. Elsevier Inc; 2006: 1-1115.
Seale SM, Feng Q, Agarwal AK, El-Alfy AT. Neurobehavioral and transcriptional effects of acrylamide in juvenile rats. Pharmacol Biochem Behav. 2012;101:77-84.
Ma Y, Shi J, Zheng M, Liu J, Tian S, He X, et al. Toxicological effects of acrylamide on the reproductive system of weaning male rats. Toxicol Ind Health. 2011;27:617-27.
Sumner SCJ, Selvaraj L, Nauhaus SK, Fennell TR. Urinary metabolites from F344 rats and B6C3F1 mice coadministered acrylamide and acrylonitrile for 1 or 5 days. Chem Res Toxicol. 1997;10:1152-60.
Friedman MA, Dulak LH, Stedham MA. A lifetime oncogenicity study in rats with acrylamide. Fundam Applied Toxicol. 1995;27:95-105.
Hammad AY, Osman ME, Abdelgadir WS. Effects of acrylamide toxicity on growth performance and serobiochemisty of Wistar rats. Br J Pharmacol Toxicol. 2013;4:163-8.
El-Mottaleb EMA, Rashed AYM. Some studies on acrylamide intoxication in male Albino rats. Egypt J Comp Path Clin Path. 2008;21:222-45.
El-Bohi KM, Moustafa GG, El sharkawi NI, Sabik LME. Genotoxic effects of acrylamide in adult male Albino rats liver. J Am Sci. 2011;7:1097-108.
Alturfan EI, Beceren A, Sehirli AO. Demiralp ZE, Sener G, Omurtag GX. Protective effect of Nacetyl-L-cysteine against acrylamide induced oxidative stress in rats Turk. J Vet Anim Sci. 2011;36:438-45.
Rawi SM, Marie MA, Fahmy SR, El- Abied SR. Hazardous effects of acrylamide on immature male and female rats. Afr J Pharm Pharmacol. 2012;6:1367-86.
Nie J, Hou FF. Role of reactive oxygen species in the renal fibrosis. Chin Med J (Engl). 2012;125(14):2598-602.
Guo ZJ, Niu HX, Hou FF, Zhang L, Fu N, Nagai R, et al. Advanced oxidation protein products activate vascular endothelial cells via a RAGE-mediated signaling pathway. Antioxid Redox Signal. 2008;10:1699-1712.
Zhou LL, Hou FF, Wang GB, Yang F, Xie D, Wang YP, et al. Accumulation of advanced oxidation protein products induces podocyte apoptosis and deletion through NADPHdependent mechanisms. Kidney Int. 2009;76:1148-60.
Wei XF, Zhou QG, Hou FF, Liu BY, Liang M. Advanced oxidation protein products induce mesangial cell perturbation through PKC-dependent activation of NADPH oxidase. Am J Physiol Renal Physiol. 2009;296:427-37.
Aluntas I, Delibas N, Sutcu R. The effects of organophosphate insecticide methidathion on lipid peroxidation and anti-oxidant enzymes in rat erythrocytes: Role of vitamins E and C. Hum Exp Toxicol. 2002;21(12):681-5.
Ayala A, Mario FM, Arguelles S. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxidative Med Cell Longevity. 2014:1-31.
Serbecic N, Beutelspacher SC. Anti-oxidative vitamins prevent lipid-peroxidation and apoptosis in corneal endothelial cells. Cell Tissue Res. 2005;320(3):465-75.
Al-Serwia RH, Ghoneim FM. The impact of vitamin E against acrylamide induced toxicity on skeletal muscles of adult male albino rat tongue: Light and electron microscopic study. J Microsc Ultrastruct. 2015;3(3):137–47.
Pace A, Savarese A, Picardo M, Maresca V, Pacetti U, Del Monte G, et al. Neuroprotecteve effect of Vitamin E supplementation in patiens treated with cisplantin chemotherapy. J Clin Oncol. 2003;21:927-31.
Rahangadale S, Kurkure N, Prajapati B, Hedaoo V, Bhandarkar AG. Neuroprotective effect of vitamin e supplementation in wistar rat treated with acrylamide. Toxicol Int. 2012;19(1):1-8.
Bancroft JD, Kim S, Christopher L. Theory and Practice of Histopathological Technique, 7th edition. Churchill, Livingstone, New York; 2012.
Kiernan J. Histological and histochemical methods, theory and practice. 3rd edition. Oxford: Butterworth-Heinemann; 2000: 320-390.
Elias JM, Newgard OF, Schorck TL. Sensitivity and efficiency of the peroxidase anti peroxidase (PAP), avidin-biotin peroxidase complex (ABC) and peroxidase-labeled avidin-biotin (LAB) method. Am J Pathol. 1989:92:62-9.
Glauert AM, Ewis PR. Biological specimen preparation for transmission electron microscopy. London: Portland Press; 1998: 17.
Dean J, Dean AJ, Coloumbier D. Epi- Info. Software computer package was on microcomputer for epidemiology, statistics and data processing. 2004, CDC, USA.
Jamshidi K. Acrylamide - induced acute nephrotoxicity in Rats. Int J Sci Res Sci Technol. 2015;1(5):286-93.
Mahmood SAF, Amin KAM, Salih SFM. Effect of Acrylamide on Liver and Kidneys in Albino Wistar Rats. Int J Curr Microbiol App Sci. 2015;4(5):434-44.
Ghorbel I, Maktouf S, Fendri N, Jamoussi K, Chaabouni ES, Boudawara T, et al. Co-Exposure to Aluminum and Acrylamide Disturbs Expression of Metallothionein, Proinflammatory Cytokines and Induces Genotoxicity: Biochemical and Histopathological Changes in the Kidney of Adult Rats. Environ Toxicol. 2016;31(9):1044-58.
Barnes JL, Gorin Y. Myofibroblast differentiation during fibrosis: role of NAD(P)H oxidases. Kidney Int. 2011;79:944-56.
Nasr AY, Saleh HA. Aged garlic extract protects against oxidative stress and renal changes in cisplatin-treated adult male rats. Cancer Cell Int. 2014;14(1):92.
Reich H, Tritchler D, Herzenberg AM, Kassiri Z, Zhou X, Gao W, et al. Albumin activates ERK via EGFR in human renal epithelial cells. J Am Soc Nephrol. 2005;16:1266-78.
Ansari MA, Raish M, Ahmad A, Ahmad SF, Mudassar S, Mohsin K. Sinapic acid mitigates gentamicin-induced nephrotoxicity and associated oxidative/nitrosative stress, apoptosis, and inflammation in rats. Life Sci. 2016;165:1-8.
Wang ET, Chen DY, Liu HY, Yan HY, Yuan Y. Protective effect of allicin against glycidamide-induced toxicity in male and female mice. Gen Physiol Biophys. 2015;34,177–87.
Stojiljković N, Ilić S, Veljković M, Jovan T. Effects of supplementation with vitamin e on gentamycin-induced acute renal failure in rats. Med Biol. 2014;16(2):61-6.
Ghlissi Z, Hakim A, Mnif H, Zeghal K, Rebai T, Sahnoun Z. Evaluation of the Protective Effect of Vitamins E and C on Acute Tubular Damage Induced by Colistin in Rat Model. Am J Phytomedici Clini Therap. 2015:3(1):43-53.
Dahdouh, F, Attalah S, Djabar MR, Kechrid Z. Effect of the joint supplementation of vitamin C and vitamin E on nickel heamatotoxicity and nephrotoxicity in male swiss albino mice. Int J Pharm Sci. 2016;8(6):234-9.
Abdel-Hamid AA, Firgany Ael-D. Vitamin E supplementation ameliorates aflatoxin B1-induced nephrotoxicity in rats. Acta Histochem. 2015;117(8):767-79.
Sanz AB, Sanchez-Nino MD, Izquierdo MC, Jakubowski A, Justo P, Blanco-Colio LM, et al. TWEAK activates the non-canonical NFkappaB pathway in murine renal tubular cells: modulation of CCL21. PLoS One. 2010;5(1):e8955.