DOI: http://dx.doi.org/10.18203/issn.2454-2156.IntJSciRep20212839

A short report on the tumor suppressor role of BIRC7 in pancreatic cancer

Aamir A. Khan, Xin H. Liu, Huan Li, Ruining Pan, Rahman U. Din, Naeem Ullah, Hua Huang

Abstract


Pancreatic cancer (PC) is accepted to be an aggressive malignancy among all type of cancers due to its poor prognosis and high cancer-led mortality ratio mostly affecting male community in older age. Multiple genes are involved in PC initiation, progression and metastasis including K-RAS, CDKN2A, p53, SMAD4. Baculoviral IAP repeat containing 7 (BIRC7) commonly known as Livin, an inhibitor of apoptosis protein (IAP) involved in the inhibition of cell death via apoptosis by preventing caspase activity through various approaches. The biological role of BIRC7 was previously identified in multiple cancers but ill investigated in PC. In this study, we investigate the function role of BIRC7 in PC. Multiple phenotypic tests including wound healing assay, CCK8 assay, trans-well assay and colony formation assay was run to rule out BIRC7 gene effect on PC genesis. We for the first time indicated that, overexpression of BIRC7 significantly reduced the proliferation, development, progression and metastasis of PANC-1 cell in vitro. Therefore, we anticipated that BIRC7 gene is a suppressor gene and might be a suitable candidate gene for therapeutic purposes in PC.


Keywords


BIRC7, Tumor suppressor, Pancreatic cancer, PADC, Gene mutation

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