Growth response and antioxidant enzyme capability of Helianthus annuus L. in lead contaminated soil under organic and urea fertilizer applications
Keywords:Helianthus annuus, Antioxidant enzymes, Detoxification, Reactive oxidative species, Fertilizers, Pb contaminated soil
Background: Indiscriminate dumping of wastes, including heavy metals such as lead (Pb) on Nigerian soils is increasing. Helianthus annuus has been found to have ability to clean up contaminated soils, but with paucity of information on the effect of Pb stress on the plant’ antioxidant enzymes activities when fertilizers are applied as soil enhancers, hence this study.
Methods: The experiment consisted of four levels (0, 400, 800, 1200 mg Pb kg-1) using [Pb (CH3COO)2.3H2O], three rates (0, 5 and 10 t ha-1) of organic and two rates (0 and 2 t ha-1) of urea fertilizers, and each treatment was replicated thrice to give a total of 72 experimental units in pot culture. Each pot contained 10 kg of sieved topsoil and arranged in a complete randomized design. The H. annuus seeds were sown, fertilizers were applied and stands of H. annuus were collected for antioxidant enzymes (SOD, CAT, POX and APX) activities determination in the roots and shoots using standard methods.
Results: Soil pH was slightly acidic and soil texture was loamy sand. Biomass yield of H. annuus increased with increase in organic fertilizer, but decreased with increase in Pb contamination. There was significant (p<0.05) increase in detoxification responses in the shoots than the roots of H. annuus against oxidative stress caused by Pb toxicity when organic fertilizer was applied to soil.
Conclusions: The study concluded that addition of organic fertilizer increased biomass yield and had superior enhancing detoxification responses on H. annuus against oxidative stress by Pb toxicity.
De Abreu CA, De Abreu MF, De Andrade JC. Distribution of Lead in the soil profile evaluated by DTPA and Mehlich-3 solutions. Bragantia. 1998:57:875-82.
Adejube AAH, Anteyi A, Garba FH, Oyekunle OA, Kudaisi FO. Bioremediating activity of sunflower (Helianthus Annuus L.) on contaminated soil from Challawa industrial area, Kano-State Nigeria. Int J Agri Earth Sci. 2017;3(5):1-11.
Yildirim E, Ekinci M, Turan M, Agar G, Ors S, Dursun A, et al. Impact of Cadmium and Lead heavy metal stress on plant growth and physiology of rocket (Eruca sativa L.) KSU J Agri Nature. 2019:22(6):843-50.
Adesodun JK, Atayese MO, Agbaje T, Osadiaye BA, Mafe O, Soretire AA. Phytoremediation potentials of sunflowers (Tithonia diversifolia and Heliathus annuus) for metals in soils contaminated with zinc and lead nitrates. Water Air soil Pollu. 2010:207:195-201.
Chaves LHG, Maria AE, Ramara SS. Effect on plant growth and heavy metal accumulation by sunflower. J Phytol. 2011:3(12):4-9.
Yanshan C, Qingren W, Yiting D, Haifeng L, Peter C. Enhanced uptake of soil Pb and Zn by Indian mustard and winter wheat following combined soil application of elemental Sulphur and EDTA. Plant Soil. 2004;261(1-2):181-8.
Adewole MB, Sridhar MKC, Adeoye GO. Removal of heavy metals from soil polluted with effluents from a paint industry using Helianthus annuus L. and Tithonia diversifolia (Hemls.) as influenced by fertilizer applications. Bioremediation Journal. 2010:14(2):169-179.
Sharma P. Dubey RS. Ascorbate peroxidase from rice seedlings: properties of enzyme isoforms, effects of stresses and protective roles of osmolytes. Plant Science. 2004:167:541-550.
Meyers DER. Auchterlonie GJ. Webb RI. Wood B. Uptake and localization of Lead in the root of Brassica juncea. Environmental Pollution. 2008;153(2):323-32.
Awotoye OO, Adewole MB, Olofinjana O. Assessment of Solenostemon monostachyus for phytoremediation potential under the influence of mycorrhuzal inoculations in heavy metal polluted soil. J Sci Res. 2011;10(2):149-59.
Bulu YI, Adewole MB. Organic fertilizer applications influence on the shoot and root biomass production and plant nutrient of Calopogonium mucunoides from crude oil-contaminated soils. Chem Speciation Bioremediation. 2015;27(1):2-7.
Souri Z, Karimi N, De Oliveira LM. Antioxidant enzymes responses in shoots of arsenic hyperaccumulator, Isatis cappadocica Desv., under interaction of arsenate and phosphate. Environ Technol. 2017;1-12.
Singh R, Tripathi RD, Dwivedi S, Kumar A, Trivedi PK, Chakrabarty D. Lead bioaccumulation potential of an aquatic macrophyte Najas indica are related to antioxidant system. Biores Technol. 2010;101:3025-32.
Gupta D, Huang H, Yang X, Razafindrabe B, Inouhe M. The detoxification of Lead in Sedum alfredii H. is not related to phytochelatins but the glutathione. J Hazard Materials. 2010;177(13):437-44.
Page AL, Miller RH, Keeney DR. Methods of soil analysis, Part 2, chemical and microbiological properties, American Society of Agronomy, Inc., Madison, WI, USA. 1982;556.
Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974;47:469-74.
Aebi H. Catalase in vitro. In Packer L. (Ed.) Methods and Enzymology, New York, Academic Press. 1984;105:121-6.
Kumar KB, Khan PA. Peroxidase and Polphenol Oxidase in Excised Ragi Eleusine coracana cv. PR 202 Leaves during Senescence. Ind J Exp Botany. 1982:20: 412-6.
Nakano Y. Asada K. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 1981;22:867-80.
Singh HB. The role of manures and fertilizers in crop production. Developing agri-input markets in Nigeria (DAIMINA). IFDC DAIMINA Market Info Bull. 2002;71.
AbdElgawad H, Zinta G, Hamed BA, Selim S, Beemster G, Hozzein WN, et al. Maize roots and shoots show distinct profiles of oxidative stress and antioxidant defense under heavy metal toxicity. Environmental Pollution. 2020;258:113705.
Goyal D, Yadav A, Prasad M, Singh TB, Shrivastav P, Ali A et al. Effect of heavy metals on plant growth: an overview. Contaminants Agri. 2020; 79-101.
Bhatt MK, Labanya R, Joshi HC. Influence of long-term chemical fertilizers and organic manures on soil fertility-A review. Universal J Agri Res. 2019;7(5):177-88.
Adugna G. A review on impact of compost on soil properties, water use and crop productivity. Academic Research J Agri Sci Res. 2016;4(3):93-104.
Wilson FEA, Harvey BMR, McAdam JH, Walton DWH. The response of white grass [Cortaderia pilosa (D’Urv.) Hack.] to nitrogen nutrition. Grass Forage Sci. 2001;56(1):84-91.
Pan B, Lam SK, Moister A, Luo Y, Chen D. Ammonia volatilization from synthetic fertilizers and its mitigation strategies: A global synthesis. Agriculture, Ecosystem Envir. 2016;232:283-9.
Gupta D, Nicoloso F, Schetinger M, Rossato L, Pereira L, Castro G, et al. Antioxidant defense mechanism in hydroponically grown Zea mays seedlings under moderate lead stress. J Hazard Materials. 2009;172(1):479-84.
Malar S, Manikandan R, Favas PJ, Sahi SV, Venkatachalam P. Effect of lead on phytotoxicity, growth, biochemical alterations and its role on genomic template stability in Sesbania grandiflora: a potential plant for phytoremediation. Ecotoxicol Envir Safety. 2014;108:249-57.
Feng-tao LI, Jian-min QI, Gao-yang Z, Li-hui L, Ping-ping F, Fen TA, et al. Effect of cadmium stress on the growth antioxidative enzymes and lipid peroxidation in two kenaf (Hibiscus cannabinus L.) plant seedlings. J Integrative Agri. 2013;12:610-20.
Oke OG. Phytoremediation potential and detoxification responses of Helianthus annuus L. in Lead contaminated soil as influenced by fertilizer applications. An MSc thesis submitted to the Postgraduate College, Obafemi Awolowo University, Ile-Ife, Nigeria. 2022;126.
Reza HH, Maliheh K, Mahlagha G. Effect of Lead on germination, growth and activity of catalase and peroxidase enzyme in root and shoot of two cultivars of Brassica napus L. J Biological Sci. 2007;7:592-8.