CYP1A1 gene polymorphism among central Indian population and its genetic distribution
Keywords:CYP1A1 gene, Polymorphism, Genetic distribution
Background: The human gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. Cytochrome P450 (CYP1A1) enzymes are involved in the oxidative metabolism of endogenous compounds such as steroids, fatty acids, leukotrienes, prostaglandins, in the metabolism of foreign chemicals such as drugs, carcinogens, and other environmental pollutants and in polycyclic hydrocarbon metabolism. This was the first study done in the region of vindhyan Madhya Pradesh.
Methods: The study population included total 300 samples from random population patients and normal healthy individuals (between 192 urban’s and 108 tribals). Physical data was collected during the sample collection.Genetic polymorphism study was carried out by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and the observed genotype frequencies, allele frequencies and P- value for CYP1A1 gene polymorphism. All the statistical analyses were performed using spss software.
Results: The genotype distribution pattern was not found different between tribal’s and urban population (χ2 =1.434 and P Value=0.4882) and overall allele frequency was also not statistically significant between two respective groups. Smoking and drinking activity data was generated through questionnaire organized during Sampling and the findings suggest that smoking have high effect over CYP1A1 gene polymorphism susceptibility.Conclusions: The genotype distribution pattern was not found different between tribal’s and urban population (χ2=1.434 and P Value=0.4882) and overall allele frequency was also not statistically significant between two respective groups.
UK Essays. Genetic Polymorphism Governing the Cyp2d6 Cytochrome P450 Enzyme Subfamily in Drug Metabolism. [Accessed 7 October 2015]. http://www.ukessays.com/dissertation/examples/sciences/genetic-polymorphism-governing-the-cyp2d6-cytrochrome-p450-enzyme-subfamily-in-drug-metabolism.php?cref=1.
Hildebrand CE, Gonzalez FJ, McBrid OW, Nebert DW. Assignment of the human 2, 4, 7, 8-tetrachlorodibenzo-p-dioxin-inducible cytochrome P1-450 gene to chromosome 15. Nucleic Acids Res. 1985;13(6):2009-16.
Gonzalez FJ, Gelboin HV. Role of human cytochromes P450 in the metabolic activation of chemical carcinogens and toxins. Drug Metab Rev 1994;26:165-83.
Nebert DW, McKinnon RA, Puga A. Human drugmetabolizing enzyme polymorphisms: effects on risk of toxicity and cancer. DNA Cell Biol. 1996;15:273-80.
Qiang MA, Lu AY. CYP1A induction and human risk assessment: an evolving tale of in vitro and in vivo studies. Drug Metab. Dispos. 2007;35:1009-16.
Jacquet M, Lambert V, Baudoux E, Muller M, Kremers P, Gielen J. Correlation between P450 CYPlAl inducibility, MspI genotype and lung cancer incidence. Eur J Cancer. 1996;32:1701-6.
Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 1988;16(3):1215.
Chaubey G. The demographic history of India: A perspective based on genetic evidence. 2010:9-81. Universitatis Tartuensis, Estonia. Ph.D Thesis. (http://hdl.handle.net/10062/15240).
Census of India [ http://www.censusindia.net/].
Thangaraj K, Naidu BP, Crivellaro F, Tamang R, Upadhyay S, Sharma VK. The influence of natural barriers in shaping the genetic structure of Maharashtra populations. 2010;PLoS One 5 e15283.
Anti-Cytochrome P450 1A1 antibody [6G5] ab126828. Product Datasheet. available at: http://www.abcam.com/Cytochrome-P450-1A1-antibody-6G5-ab126828.tml
Garte S, Gaspari L, Alexandrie S, Ambrosone C, Autrup H, Autrup J. Metabolic Gene Polymorphism Frequencies in Control Populations. Cancer Epidemiol Biomarkers. 2001;10:1239.