CYP1A1 gene polymorphism among central Indian population and its genetic distribution

Abstract

Background: The human gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. Cytochrome P450 (CYP1A1) enzymes are involved in the oxidative metabolism of endogenous compounds such as steroids, fatty acids, leukotrienes, prostaglandins, in the metabolism of foreign chemicals such as drugs, carcinogens, and other environmental pollutants and in polycyclic hydrocarbon metabolism. This was the first study done in the region of vindhyan Madhya Pradesh.

Methods: The study population included total 300 samples from random population patients and normal healthy individuals (between 192 urban’s and 108 tribals). Physical data was collected during the sample collection.Genetic polymorphism study was carried out by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and the observed genotype frequencies, allele frequencies and P- value for CYP1A1 gene polymorphism. All the statistical analyses were performed using spss software.

Results: The genotype distribution pattern was not found different between tribal’s and urban population (χ2 =1.434 and P Value=0.4882) and overall allele frequency was also not statistically significant between two respective groups. Smoking and drinking activity data was generated through questionnaire organized during Sampling and the findings suggest that smoking have high effect over CYP1A1 gene polymorphism susceptibility.

Conclusions: The genotype distribution pattern was not found different between tribal’s and urban population (χ2=1.434 and P Value=0.4882) and overall allele frequency was also not statistically significant between two respective groups.