DOI: http://dx.doi.org/10.18203/issn.2454-2156.IntJSciRep20163394

Evaluation of phytochemical and pharmacological activities of Bacopa monnieri (L.)

Amitabh Basak, Md. Lokman Hossain, Most. Nazma Parvin

Abstract


Background: Bacopa monnieri (L.) locally called Brahmi of Scrophulariaceae family has a long history for numerous therapeutic purposes like memory enhancing, antianxiety and antiepileptic agent. The aim of this study was to investigate phytochemical screening, antibacterial, cytotoxic, analgesic and neuropharmacological activities of B. monnieri.

Methods: The antibacterial activity was performed by disc diffusion method.  Brine shrimp lethality bioassay was carried out to determine cytotoxic potential. Acetic acid induced writhing method was employed for the assessment of analgesic activity of B. monnieri extracts. The neuropharmacological activity was determined by hole cross, open field and thiopental sodium induced sleeping time test using Swiss Albino mice as experimental animal.

Results: Phytochemical screening revealed that n-hexane, dichloromethame and methanolic extracts contained reducing sugar, alkaloids, flavonoids, steroids and saponins. The sample showed comparable antimicrobial and cytotoxic activity. In analgesic activity test, methanol soluble extract showed highest activity compared to standard drug, Diclofenac sodium. The neuropharmacological activity of three extracts showed moderate activity as compared with standard drug, Diazepam and Thiopental sodium.

Conclusions: Our exploration suggests that B. monnieri contains bioactive compounds and it should be studied further for isolation and purification of such novel compounds.

 


Keywords


Bacopa monnieri, Antibacterial, Cytotoxicity, Analgesic, Neuropharmacological

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References


Ghani A. Medicinal Plants of Bangladesh. Dhaka: Asiatic Society of Bangladesh; 1998.

Chopra RN. Indigenous Drugs of India. 2nd ed. Calcutta, India: U.N. Dhur and Sons; 1958: 341.

Sarkar KK, Hossain ML, Hossin A, Mondal H, Monjur-Al-Hossain ASM, Ahmed MI, et al. Pharmacological studies on Glycosmis pentaphylla (corr.) whole plant. Pharmacology Online. 2013;1:230-6.

Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. London: Chapman and Hall; 1998: 182-190.

Bauer AW, Kirby WMM, Sherris KC, Turk M. Antibiotic susceptibility testing by standardized singledisc method. Am J clin patho. 1966;45:493-6.

Persoone G, Vanhaecke P, Claus C, Sorgeloos P. Proposal for a short-term toxicity test with Artemia nauplii. Ecotoxicol Env Safety. 1981;5:382-7.

Takagi K, Watanabe M, Saito H. Studies on the spontaneous movement of animals by the hole cross test: Effect of 2-dimethylaminoethane. It’s acylates on the central nervous system. Jpn. J. Pharmacol. 1971;21:797-810.

Gupta BD, Dandiya PC, Gupta ML. A psychopharmacological analysis of behavior in rat. Jpn J Pharmacol. 1971;21:293-8.

Sabina EP, Chandel S, Rasool MK. Evaluation of analgesic, antipyretic and ulcerogenic effect of withaferin A. Int J Integr Biol. 2009;6:52-6.

Leach FS. Anti-microbial properties of Scutellaria baicalensis and Coptis chinensis, two traditional Chinese medicines. Bioscience Horizons. 2011;4:119-27.

Ozcelik B, Kartal M, Orhan I. Cytotoxicity, antiviral and antimicrobial activities of alkaloids, flavonoids, and phenolic acids. Pharm Biol. 2011;49:396–402.

Ahmadiani A, Hosseiny J, Semnanian S, Javan M, Saeedi F, Kamalinejad M, et al. Antinociceptive and anti-inflammatory effects of Elaeagnus angustifolia fruit extract. J Ethnopharmacol. 2000;72:287-92.

Narayana KR, Reddy MS, Chaluvadi MR. Bioflavonoids Classification, Pharmacological, Biochemical effects and Therapeutic potential. Indian J Pharmacol. 2001;33:2-16.

Hall CS. Emotional behavior in the rat. I. Defecation and urination as measures of individual differences in emotionality. J. Comp. Psychol. 1934;18:385-403.

Hall CS. Emotional behavior in the rat. III. The relationship between emotionality and ambulatory activity. J. Comp. Psychol. 1936;22:345-52.

Broadhurst PL. The Maudsley reactive and nonreactive strains of rats: A survey. Behav. Genet. 1957;5:299-319.

DeFries JC, Wilson JR, McClearn GE. Open-field behavior in mice: Selection response and situational generality. Behav. Genet. 1970;1:195-211.

DeFries JC, Hegmann JP, Halcomb RA. Response to 20 generations of selection for open-field activity in mice. Behav. Biol. 1974;11:481-95.

DeFries JC, Gervais MC, Thomas EA. Response to 30 generations of selection for open-field activity in laboratory mice. Behav. Genet. 1978;8:3–13.

Blizard DA. The Maudsley reactive and nonreactive strains: A North American perspective. Behav. Genet. 1981;11:469-89.