Published: 2017-01-26

Effect of long term consumption of graded levels of powdered stored Ocimum gratissimum supplementation on the weight performance in Wistar rats

Ogungbemi Kunle, Ishola D. Taiwo, Ishola O. Tomilayo, Arowora K. Adebisi, Ilesanmi F. Funmilayo


Background: The influence of Ocimum gratissimum leaf supplementation on feed intake; weight change via growth performance of Wistar rats was investigated.

Methods: A total of twenty (20) rats (average weight of 80 g±10 g), housed in a well-ventilated cages and given feed and water ad libitum for 2 weeks after which they were randomly allotted to  four dietary treatments comprising 0%, 2.5%, 5% and 10%, of powdered stored Ocimum gratissimum leaf inclusion.  

Results: Group of rats fed with the lowest supplementation (2.5%) of Ocimum gratissimum showed significant increase (P< 0.05) in weekly feed intake than group fed with the highest supplementation (10%). While, there was no significant difference (P >0.05) between the groups fed with 0% and 5%. Also, the weight gain by the rats with 2.5% Ocimum gratissimum diet supplementation had highest weight gain which was significantly different to 10% supplementation and non-significantly different to 5% supplementation. Animals which received 2.5% of leaf of Ocimum gratissimum had the fastest growth rate, followed by those that received 5% and 10%. However, the control feed had higher body weight gain than 10% supplementation. The observable increase in the feed intake, weight gain and feed efficiency seen in the lowest supplementation level (2.5%) can be attributed to the spicing and seasoning of food with O. gratissimum and as such could also increase the palatability of the food. Furthermore, 2.5% has the highest dietary lipid which increases the palatability of food by absorbing and retaining flavor.

Conclusions: It can be concluded that 2.5% O. gratissimum supplementation has a better palatability than other experimental diets which reflected on the performance characteristics of this treatment. 


Ocimum gratissimum, Feed intake, Feed efficiency, Weight performance, Wistar rat

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Okafor JC. Edible indigenous woody plants in the rural economy of the Nigerian forest zone. Forest Ecol Manag. 1980;3:45-55.

Okigbo RN, Ogbonnaya UO. Antifungal effects of two tropical plant leaf extracts (Ocimum gratissimum and Aframomum melegueta) on postharvest yam (Dioscorea spp.) rot. African J Biotechnol. 2006;5(9):727-31.

Okujagu TF, Etatuvie SO, Eze I, Iimoh B, Nwokereke C, Mbaoji C, et al. Medicinal Plants of Nigeria (South West Zone Vol. 1). Nigeria Natural Medicine Development Agency, Federal Ministry of Science and Technology, Lagos, Nigeria. 2008: 204.

Odebiyi OO, Sofowora EA. Phytochemical screening of Nigerian medicinal plants II. Lloydia. 1977;41(3):234-46.

Iwu MM. Empirical investigations of dietary plants used in Igbo ethnomedicine. Plants in indigenous medicine & diet: biobehavioral approaches. Bedford Hills: Redgrave; 1986: 131-150.

Okigbo BN. Neglected plants of horticultural and nutritional importance in traditional farming systems of tropical Africa. In IV Africa Symposium on Horticultural Crops. 1975;53:131-50.

Aguiyi JC, Obi CI, Gang SS, Igweh AC. Hypoglycaemic activity of Ocimum gratissimum in rats. Fitoterapia. 2000;71(4):444-6.

Yan L, Yee JA, Li D, McGuire MH, Thompson LU. Dietary flaxseed supplementation and experimental metastasis of melanoma cells in mice. Cancer letters. 1998;124(2):181-6.

AOAC. Official Mmethods of Aanalysis 18th Association of Official Analysis Chemist Washington D.C. 2005: 774-784.

Pearson D. The chemical analysis of foods. 7th edition. Longman Group Ltd; 1976.

Achnewhu SC, Ogbonna CC, Hart AO. Chemical composition of indigenous wild herbs, spices, fruits, nuts, and leafy vegetable used as food. J Plant Foods for Human Nutrition. 1995;4(8):341-8.

Adepoju OT, Oyewole EO. Nutritional importance and Micronutrients potentials of non-conventional indigenous green leafy vegetable from Nigeria. Agricultural J. 2008;3(5):362-5.

Edeoga HO, Omosun G, Uche LC. Chemical composition of Hyptissuaveolens and Ocimum gratissimum hybrids from Nigeria. African J Biotechnol. 2006;5(10):892-5.

Antial BS, Akpang EJ, Okonl PA, Umorenl IU. Nutritive and Antinutritive Evaluation of Sweet potatoes (Ipomeabatata) Leaves. Pak J Nut. 2006;5(2):166-8.