DOI: http://dx.doi.org/10.18203/issn.2454-2156.IntJSciRep20170359

Prevalence and drug susceptibility pattern of Shigella and Salmonella species in under ten diarrhoeic children admitted to Tirunesh-Beijing hospital

Habtamu Hawaz, Selamawit Girma, Yordanos Tezera, Umer Ahmed, Musin Kelel

Abstract


Background: Diarrheal diseases constitute a major burden of disease in the low and middle-income countries of the world with diarrhea being the second leading cause of morbidity. Now a day, food borne disease, caused by Shigella and Salmonella species is the most common type of bacterial gastroenteritis  have gained multiple antimicrobial resistances; the challenge for clinical management. For acute childhood diarrhea that requires antimicrobial therapy correct choice of the drug depends on detailed previous knowledge of local strains. Therefore, the objective of the study was to assess the prevalence and drug susceptibility pattern of diarrheal diseases in under 10 children admitted to Tirunesh-Beijing hospital to provide preliminary information for healthcare providers.

Methods: Selective and differential media for isolation of diarrhea causing bacterial species was used. Further biochemical tests were conducted to confirm the results. The drug sensitivity and resistance pattern of isolated bacteria was assessed by minimum inhibitory concentration (MIC) as well as minimum bactericidal concentration (MBC). Data about patients' sex and age, pathogens isolated and their antimicrobial resistance patterns were recorded.  

Results: Out of 22 stool samples collected from diarrhoeic under ten children the majority was males. Two (9.1%) stool samples were found positive for one Shigella sonnei and one Shigilla flexnrei while no Salmonella species was identified.

Conclusions: The isolated Shigella species showed high antibiotic resistance to amoxicillin, cotrimoxazole and ampicillin. But it was susceptible to ciprofloxacin. Therefore, frequent assessment of the pattern of resistance and prevalence is needed to keep the community save and use appropriate drug for treatment in the study area. 


Keywords


Diarrhea, Antimicrobial susceptibility, Salmonella, Shigella, Children

Full Text:

PDF

References


World Health Organization. Diarrhoeal disease due to Shigella disease. In: Vaccines, immunization and biologicals. World Health Organization bulletin. 1998;8:1-5.

Ahs JW, Tao W, Lofgren J, Forsberg BC. Diarrheal diseases in low and middle-income countries: Incidence, prevention and management. Open Infect Dis J. 2010;4:113-24.

Seidlein VL, Ali KM, Lee H, Wang X, Thiwm VD. A multicenter study of shigella diarrhea in six Asian countries: disease burden, clinical manifestations, and microbiology. Plos Med. 2006;3:363.

Jesudason MV, Lalitha MK, Koshi G. Changes in incidence of shigella subgroups and their antibiotic susceptibility pattern in Vellore, south India. J Trop Med Hyg. 1985;88:355-8.

Tjaniadi P, Lesmana M, Subekti D. Antimicrobial resistance of bacterial pathogens associated with diarrheal patients in Indonesia. Am J Trop Med Hyg. 2003;68:666-70.

Munshi M, Haider K, Rahaman M, Sack D, Ahmed Z, Morshed M. Plasmid-mediated resistance to nalidixic- acid in Shigelladysenteriae type 1. Lancet. 1987;330:419-21.

Ashkenazi S, May ZM, Sulkes J, Zilberberg R, Samra Z. Increasing antimicrobial resistance of Shigella isolates in Israel during the period 1984 to 1992. Antimicrob Agents Chemother. 1995;39:819-23.

Niemogha MT, Alabe SA, Uzoma KL. The incidence of Salmonella, Shigella and other enteric bacterial pathogens in stool specimens of diarrhoea patients. Niger Med J. 1995;28:70-4.

Mekonnen H, Kebede A, Menkir S. Isolation rate and drug resistance patterns of Shigella species among diarrheal patients attending at Hiwot Fana Hospital, Harar, Ethiopia. Ethiop J Sci Technol. 2014;7(1):15-25.

Andargie G, Kassu A, Moges F, Tiruneh M, Huruy K. Prevalence of bacteria and intestinal parasites among food-handlers in Gondar Town, Northwest Ethiopia. J Health Popul Nutr. 2008;26:451-5.

Getameasy M, Getnet B, Ahmed Z. Prevalence of shigella, salmonella and cmpylobacter species and their susceptibility patters among under five children with diarrhea in hawassa town, south Ethiopia. Eth J Health Sci. 2014;24(2):101-8.

Jemal WY, Rotimi VO, Chugh TD, Pal T. Prevalence and susceptibility of Shigella species to 11 antibiotics in a Kuwait teaching hospital. J Chemother. 1998;15:10285-90.

Mardaneh J, Poor SA, Afrugh P. Prevalence of Shigella species and Antimicrobial Resistance Patterns of Isolated Strains from Infected Pediatrics in Tehran. Int J Enterpathog. 2013;1:28-31.

Tiruneh M. Serodiversity and antimicrobial resistance pattern of Shigella isolates at Gondar University teaching Hospital, Northwest Ethiopia. Jpn J Infect Dis. 2009;62:93-7.

Ahmed K, Shakoori FR, Shakoori AR. Aetiology of shigellosis in northern Pakistan. J Health Popul Nutr. 2003;21:32-9.