Comparative evaluation of efficacy of various probiotics on Streptococcus species


  • Shweta Chandak Department of Pedodontics and Preventive Dentistry, SDKS Dental College and Hospital, Nagpur, Maharashtra
  • Ankita A. Moon Department of Pedodontics and Preventive Dentistry, SDKS Dental College and Hospital, Nagpur, Maharashtra
  • Shruti S. Karale Department of Pedodontics and Preventive Dentistry, SDKS Dental College and Hospital, Nagpur, Maharashtra
  • Amit Bharadwaj Department of Orthodontics, Modern Dental College, Indore, Madhya Pradesh



Probiotics, Streptococcus mutans, Streptococcus sanguinis, Streptococcus sobrinus


Background: Probiotics which when administered in adequate amounts confer a health benefit on the host. The role of probiotics is the replacement of pathogenic species with non-pathogenic species. Dairy food like cheese, curd and milk are considered as useful vehicles to carry probiotic bacteria. Aim of the study was to compare and evaluate the efficacy of various probiotics against different Streptococcus species.

Methods: Three probiotic products viz probiotic milk, probiotic yogurt and probiotic capsules were used. Streptococcus species i.e. S. mutans, S. sanguinis and S. sobrinus were isolated from the saliva of children with moderate to high caries. 0.2 ml of each probiotic product was transferred to the blood agar plates coated with Streptococcus species.  

Results: The zone of inhibition was observed in all the test groups, for all the Streptococcus species against all the probiotics, highest for S. mutans against probiotic milk. The growth of S. mutans, S. sanguinis and S. sobrinus was inhibited by L. casei shirota present in probiotic milk (Yakult), Lactobacilli acidophilus present in probiotic yogurt (Actiplus nestle) followed by Lactobacilli rhamnosus present in pre and probiotic capsule (Inlife).

Conclusions: The use of probiotic products that are readily available and cost effective like milk and yogurt can be inculcated in general population especially in children as a preventive tool for dental caries. 


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Author Biography

Ankita A. Moon, Department of Pedodontics and Preventive Dentistry, SDKS Dental College and Hospital, Nagpur, Maharashtra

Post Graduate student


Guarner F, Perdigon G, Corthier G, Salminen S, Koletzko B, Morelli L. Should yoghurt cultures be considered probiotic? Br J Nutr. 2005;93:783-6.

Metchnikoff E. The prolongation of life. In: Heinemann W, editor. Optimistic Studies. London: G.P. Putnamand Sons; 1907: 1-100.

Meurman JH, Stamatova I. Probiotics: Contributions to oral health. Oral Dis. 2007;13:443-51.

Vishnu HP. Probiotics and oral health. In: Virdi M, editor. Oral Health Care: Pediatric Research, Epidemiology and Clinical Practices. Bangalore, India: Vydehi Institute of Dental Sciences and Research Centre; 2012: 195‑204.

Meurman JH. Probiotics: do they have a role in oral medicine and dentistry? Eur J Oral Sci. 2005;113:188-96.

Islam, B, Khan, SN, Khan, AU. Dental caries: From infection to prevention. Med Sci Monit. 2007;13:196-203.

Ramakrishna, Y, Goda, H, Baliga, MS, Munshi, AK. Decreasing cariogenic bacteria with a natural, alternative prevention therapy utilizing phytochemistry (plant extracts). J Clin Pediatr Dent. 2011;36:55-63.

Cannon M, Trent B, Vorachek A, Kramer S, Esterly R. Effectiveness of CRT at Measuring the Salivary Level of Bacteria in Caries Prone Children. J Clin Pediatr Dent. 2013;38(1):55-61.

Ge Y, Caufield PW, Fisch GS, Li Y. Streptococcus mutans and Streptococcus sanguinis colonization correlated with caries experience in children. Caries Res. 2008;42:444-8.

Oda Y, Hayashi F, Okada M. Longitudinal study of dental caries incidence associated with Streptococcus mutans and Streptococcus sobrinus in patient with intellectual disabilities. BMC Oral Health. 2015;15(102):1-5.

Bhalla M, Ingle NA, Kaur N, Yadav P. Mutans streptococci estimation in saliva before and after consumption of probiotic curd among school children. J Int Soc Prevent Communit Dent. 2015;5:31-4.

Poureslami H, Pishbin L, Eslaminejad Z, Jahani Moqadam F, Rashid Farokhi M. The effects of a dairy probiotic product, espar, on salivary calcium and mutans streptococci. J Dent Res Dent Clin Dent Prospects. 2013;7(3):147-51.

Saavedra JM, Abi-Hanna A, Moore N, Yolken RH. Long-term consumption of infant formulas containing live probiotic bacteria: tolerance and safety. Am J Clin Nutr. 2004;79:261–7.






Original Research Articles