Regulatory mechanisms associating innate leaves senescence of incongruent species
Background: Senescence is the final developmental phase of a leaf which starts with nutrient salvage and ends with cell death. The first visible event during senescence is leaf yellowing, which typically starts at the leaf margins and progresses to the interior of the leaf blade. Though, regulators of senescence adopt a range of physiological and developmental mechanisms which undergo senescence of plant.
Methods: Leaves of different species were collected from the green house, and then rinsed several times with sterilized distilled water. For discs of leaves, two same sized leaves were collected and made the same sized discs. The samples were infiltrated with specific senescence inhibitor. The discs then kept in distilled water and placed under condition at 250 C. Observed the phenotypes at two days interval, molecular based analysis was perfumed at 8th day of infiltration.
Results: In this study, innate senescence approach comparison to inhibitor based senescence has been performed in order to check its consequences on leaves of different crops such as; cauliflower, apple, tobacco, rose and Arabidopsis. Arabidopsis and apple have resulted in a narrative phenotype with high level of ion leakage. While in case of rose and cauliflower, the phenotype was characterized with yellow fading of leaves. Interestingly, in the tobacco plants, intense yellowing of leaves developed along bottom. Further, in order to confirm the efficiency and pattern of senescence, we had also assessed the changes occurred during leaf senescence via ion leakage and chlorophyll content, expression of SAG12 (a senescence associated gene) and (PSA) photosynthetic associated genes expression as markers.
Conclusions: It has been noted that progression of leaf senescence is a very critical and important factors affecting plant growth and development. It can be stated that initiation of leaves senescence can be controlled by using specific inhibitor.
Quirino BF, Noh YS, Himelblau E, Amasino RM. Molecular aspects of leaf senescence. Trends in Plant Science. 2000;5:278-82.
Lim PO, Kim HJ, Nam HG. Leaf senescence. Annual Rev Plant Biol. 2007;58:115-36.
Chen W, Provart NJ, Glazebrook J. Expression profile matrix of Arabidopsis transcription factor genes suggests their putative functions in response to environmental stresses. Plant Cell. 2002;14:559–574.
Guo Y, Cai Z, Gan S. Transcriptome of Arabidopsis leaf senescence. Plant, Cell Environ. 2004;27:521–49.
Lin JF, Wu SH. Molecular events in senescing Arabidopsis leaves. Plant J. 2004;39:612–28.
Buchanan-Wollaston V, Page T, Harrison E. Comparative transcriptome analysis reveals significant differences in gene expression and signaling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005;42:567–85.
Balazadeh S, Rian-o-Pacho DM, Mueller-Roeber B. Transcription factors regulating leaf senescence in Arabidopsis thaliana. Plant Biol. 2008;10:63–75.
Buchanan-Wollaston V, Earl S, Harrison E, Mathas E, Navabpour S, Page T, Pink D. The molecular analysis of leaf senescence: a genomics approach. Plant Biotechnol J. 2003;1:3-22.
Gan S, Amasino RM. Making sense of senescence Molecular genetic regulation of leaf senescence. Plant Physiol. 1997;113:313–9.
Nagamatsu A, Masuta C, Senda M, Matsuura H, Kasai A, Hong JS, et al. Functional analysis of soybean genes involved in flavonoid biosynthesis by virus-induced gene silencing. Plant Biotechnol J. 2007;5:778–90.
Gan H, Chang J, Feng JJ, Hu HH. Direct numerical simulation of the sedimentation of solid particles with thermal convection. J Fluid Mech. 2003;481:385–411.
Hensel LL, Grbic V, Baumgarten DA, Bleecker AB. Developmental and age-related processes that influence the longevity and senescence of photosynthetic tissues in Arabidopsis. Plant Cell. 1993;5:553–64.
Noodén LD, Penney JP. Correlative controls of senescence and plant death in Arabidopsis thaliana (Brassicaceae). J Exp Bot. 2001;52(364):2151-9.
Jing YP, Börner G, Suto Y. Spatial Correlation Functions and the Pairwise Peculiar Velocity Dispersion of Galaxies in the Point Source Catalog Redshift Survey: Implications for the Galaxy Biasing in Cold Dark Matter Models. Astrophysical J. 2002;1(564):15-22.
Levey S and Wingler A. Natural variation in the regulation of leaf senescence and relation to other traits in Arabidopsis. Plant, Cell Environ. 2005;28:223–31.
Ayumi T, Amane M. Photosynthetic Research in Plant Science. Plant Cell Physiol. 2009;50(4):681–3.
Hee K, Junyoung K, Jeongsik K, Ung L, In-Ja S, Jin-Hong K, et al. The RAV1 transcription factor positively regulates leaf senescence in Arabidopsis. J Experiment Botany. 2010;61(14):3947–57.
Buchanan-Wollaston V, Page T, Harrison E. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005;42:567-85.
Andersson A, Keskitalo J, Sjödin A. A transcriptional timetable of autumn senescence. Genome Biol. 2004;5:24.
Oh SA, Park JH, Lee GI, Paek KH, Park SK, Hong GN. Identification of three genetic loci controlling leaf senescence in Arabidopsis thaliana. Plant J. 1997;12:527–35.
Burch-Smith TM, Anderson JC, Martin GB, Dinesh-Kumar SP. Applications and advantages of virus-induced gene silencing for gene function studies in plants. Plant J. 2004;39:734–46.
Lin Z, Hong Y, Yin M, Li C, Zhang K, Grierson D. A tomato HD-Zip homeobox protein, LeHB-1, plays an important role in floral organogenesis and ripening. Plant J. 2008;55:301–10.
Lim PO, Woo HR, Nam HG. Molecular genetics of leaf senescence in Arabidopsis. Trends Plant Sci. 2003;8:272–8.
Zentgraf U, Jobst J, Kolb D, Rentsch D. Senescence-related gene expression profiles of rosette leaves of Arabidopsis thaliana:leaf age versus plant age. Plant Biol. 2004;6:178-83.
Fu DQ, Zhu BZ, Zhu HL, Zhang HX, Xie YH, Jiang WB, et al. Enhancement of virus-induced gene silencing in tomato by low temperature and low humidity. Mol Cells. 2006;21:153–60.
Dinesh-Kumar SP, Anandalakshmi R, Marathe R, Schiff M, Liu Y. Virus-induced gene silencing. Methods Mol Biol. 2003;236:287–94.