Phytochemical screening, antinociceptive, anthelmintic and cytotoxicity studies of the leaves of Carissa carandas Linn. (Family: Apocynaceae)
Keywords:Carissa carandas, Phytochemical screening, Antinociceptive, Anthelmintic, Cytotoxicity
Background: Carissa carandas Linn. (Bengali name- Karamya, Karancha, Karamcha, English name-Karaunda) commonly known as Karanda is a widly used medicinal plants belongs to apocynaceae family. The various plant parts of C. carandas have been used for ethnomedicine in the treatment of human diseases, such as diarrhea, stomachic, anorexia, intermittent fever, mouth ulcer and sore throat, syphilitic pain, burning sensation, scabies and epilepsy. The current studies were aimed to investigate the phytochemical constituents, antinociceptive, anthelmintic and cytotoxic activities of the methanol extract of leaves of C. carandas.
Methods: The antinociceptive property of the methanol leaves extract of C. carandas was evaluated in swiss albino mice by using acetic acid-induced writhing test and anthelmintic activity of the fresh leaves juice was performed by observing the time of paralysis and the time of death of earth worms Pheretima posthuma. On the other hand, the methanol extract of leaves of C. carandas was screened for cytotoxic activity by brine shrimp lethality bioassay.
Results: Phytochemical screening showed that the methanol leaves extract contained alkaloids, steroids, flavanoids, tannins, saponins and reducing sugar. The antinociceptive activity test revealed that the extract showed significant antinociceptive activity and the fresh juice of the leaves of C. carandas showed potent anthelmintic activity. In cytotoxicity studies, the methanol leaves extract displayed moderate cytotoxic activity when compared with standard drug, vincristine sulphate.
Conclusions: In the current studies, these observations also support the use of this plant for medicinal purposes and encourage further investigations for more fruitful results.
Ghani A. Medicinal Plants of Bangladesh: Chemical Constituents and Use. The Asiatic Society of Bangladesh, Dhaka. 2003;2:149-50.
Devmurari V, shivanand P, Jivani NP. A review on Carissa congesta: Phytochemical constituents, traditional use and pharmacological properties. Pharmacognosy Rev. 2010;8(1):81-2.
Shinde M, Chaudhuri S, Zaware B. Antianxiety activity of Carissa congesta weight leaves. J Pharm Res. 2016;10(12):825.
Singh A, Uppal GK. A review on Carissa carandas-phytochemical, ethno-pharmacology and micropropagation as conservation strategy. Innovare Acad. 2015;8(3):26.
Hasan SMR. Phytochemical and pharmacological investigations of Commelina benghalensis Linn. M. Pharm. Thesis. Department of Pharmacy, Jahangirnagar University. 2009.
Ghani A. Medicinal Plants of Bangladesh with Chemical Constituents and Uses. Asiatic Society of Bangladesh, Dhaka. 1998;2:4-19.
Rang HP, Dale M. M. Pharmacology. Churchill Livingstone Publisher, UK. 1993;2:706-11.
Tripathi KD. Essentials of Medical Pharmacology. Jaypee Brothers Medical Publishers (P) Ltd., New Delhi, India. 1999;4:432.
Meera S, Kumar NS, Guptatyam VSS. Screening of Anti-Arthritic, Anti-Inflammatory and Analgesic Activity of a Polyherbal Formulation. Int J Pharmacol. 2008;4(5):398–402.
Parvin MN. Cytotoxicity, analgesic and anthelmintic studies of the different plant parts of Saraca indica Linn. (Family: Caesalpiniaceae). Int J Sci Rep. 2016;2(11):278-83.
Mayer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, Mclaughlin JL. Brine shrimp: A convenient bioassay for active plant constituents. Plan. Med. 1982;45:31-4.
Sabina EP, Chandel S, Rasool MK. Evaluation of analgesic, antipyretic and ulcerogenic effect of A. withaferin. Int J Integr Biol. 2009;6:52-6.
Ahmadiani A, Hosseiny J, Semnanian S, Javan M, Saeedi F, Kamalinejad, M,Saremi S. Antinociceptive and anti-inflammatory effects of Elaeagnus angustifolia fruit extract. J Ethnopharmacol. 2000;72:287-92.
Narayana KR, Reddy MS, Chaluvadi MR. Bioflavonoids Classification, Pharmacological, Biochemical effects and Therapeutic potential. Indian J Pharmacol. 2001;33:2-16.
Mishra CK, Sasmal D, Shrivastava B. An in vitro evaluation of the anthelmintic activity of unripe fruits extract of Carissa carandas Linn. Int J Drug Dev Res. 2012;4(4):393-7.
Ozcelik B, Kartal M, Orhan I. Cytotoxicity, antiviral and antimicrobial activities of alkaloids, flavonoids, and phenolic acids. Pharm Biol. 2011;49:396–402.