Black Sea fish and shellfish as essential source of vitamin B12
DOI:
https://doi.org/10.18203/issn.2454-2156.IntJSciRep20183181Keywords:
Cyanocobalamin, UV-Vis, Spectrometry, Cobalt-complexes, MusselAbstract
Background: Vitamins are very important for the human body because they are necessary for many biological absorption processes of other nutrients, for cells and tissues growth and repair. Because of this, there are many recommendations on daily vitamins intake, approved by the different country and world food organizations. Usually under the term "vitamin B12" is understood only cyanocobalamin, but actually this name is general and covers all potentially biologically active "cobalamins"- a group of cobalt-containing compounds. Animal foods, as different meat, milk, eggs, fish and shellfish are considered as the main dietary sources of this vitamin. They contain different forms (methylcobalamin and deoxyadenosylcobalamin) of vitamin B12 in different amounts. There is limited information in the scientific literature about the vitamin B12 content in black sea fish and shellfish. The aims of the present work were to determine and compare vitamin B12 contents as well as relative daily intake of vitamins in different fish and shellfish species from Black Sea waters.
Methods: Vitamin B12 was analysed spectrophotometrically. The method for quantitative analysis includes extraction from the edible tissue and enzymatic hydrolysis to release the cobalt ions.
Results: The quantities of vitamin B12 found in the edible tissue of the analysed samples ranged from 0.63 μg.100 g-1 ww to 21.5 μg.100 g-1 ww.
Conclusions: The observed results confirm that all fish and molluscs samples deliver significant quantities of the water-soluble vitamin B12.
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References
Watanabe F. Vitamin B12 Sources and Bioavailability. Exp Biol Med. 2007;232:1266–74.
O’Leary F, Samir S. Vitamin B12 in Health and Disease. Nutrients. 2010;2:299-316.
Scott J. Bioavailability of vitamin B12. Eur J Clin Nutr. 1997;51(1):49-53.
Watanabe F, Bito T. Vitamin B12 sources and microbial interaction. Exp Biol Med (Maywood). 2018;243(2):148-58.
Farquharson J, Adams J. The forms of vitamin B12 in foods. Br J Nutr. 1976;36(1):127-36.
Food and Nutrition Board: Institute of Medicine. Vitamin B12. In Dietary reference intakes for thiamin, riboflavin, niacin, vitamin B6, folate, vitamin B12, pantothenic acid, biotin and choline. National Research Council, Ed. Washington, DC, USA: National Academy Press; 1998: 306-356.
Ribarova F. Food and vitamins. Sofia, Bulgaria 2007.
Weir DG, Scott JM. Cobalamins physiology, dietary sources and requirements. In: Sadler M, Strain JJ, Caballero B, eds. Encyclopedia of human nutrition. Volume 1. San Diego, CA: Academic Press; 1998;394–401.
Hill HAO, Pratt JM, Thorp RG, Ward B, R. Williams JP. The Chemistry of Vitamin B12 - the co-ordination of biologically important molecules. Biochem J. 1970;120:263-9.
Karmi O, Zayed A, Baraghethi S, Qadi M, Ghanem R. Measurement of vitamin B12 concentration: a review on available methods. II OAB-India. 2011;2(2):23-32.
Lebiedzinska A, Marszall ML, Kuta J. Reversed phase high performance liquid chromatography method with coulometric electrochemical and ultraviolet detection for the quantification of vitamins B1, B6 and B12 in animal and plant foods. J Chromatogr A. 2007;1173:71-80.
Heudi O, Kilinc T, Fontannaz P, Marley E. Determination of vitamin B in food products and in premixes by reversed-phase high performance liquid chromatography and immunoaffinity extraction. J Chrom A. 2006;1101:63-8.
Divarova V, Racheva P, Lekova V, Gavazov K, Dimitrov A. Spectrophotometric determination of cobalt(II) in a liquid-liquid, extraction system containing 4-(2-thiazolylazo) resorcinol and 2,3,5-triphenyl-2h-tetrazolium chloride. J Chem Tech Met. 2013;48(6):623-30.
Chohan ZH, Farooq MA. Spectrophotometric Determination of Cobalt(II) Using N-(2-pyrrolylmethylene)- 2,4, 6-triamino-1,3,5- triazine as a Chromogenic Reagent. J Chem Soc Pak. 2001;23(3):168-70.
Eskandari H, Ghaziaskar HS, Ensafi AA. A sensitive and simple extractive-Spectrophotometric method for the determination of microgram amount of cobalt by using a-Benzilmonoxime. Anal Sci. 2001;17:327-31.
Ahmadnia A, Sahari MA, Barzegar M, Seyfabadi SJ, Abdollahi M. Vitamins Contents of some Commercially Important Fish Species from South Caspian Sea. Am-Eur J Sust Agr. 2008;2(3):285-293.
Whole Food Catalog, 2010. Available at: http://wholefoodcatalog.info/nutrients/. Accessed on 21 May 2016.
USDA Food Composition Databases, 2018. Available at: https://ndb.nal.usda.gov/ndb/ nutrients/index. Accessed on 20 May 2018.
Santhanam R. Nutritional Marine Life. CRC Press 15.12.2014.
Ordinance no 1 / 22.01.2018 г., Ministry of health, the physiological norms of nutrition of the population. Available at: http://www.mh. government.bg/media/filer_public/2018/02/13/naredba1-22-01-2018-fiziologicni-normi-hranene-naselenie.pdf. Accessed on 20 May 2018.