Integrated OF-HC-EPM approach to assess sedative-anxiolytic potential of honey derived from mustard (Brassica nigra) flower
DOI:
https://doi.org/10.18203/issn.2454-2156.IntJSciRep20193206Keywords:
Mustard honey, Open field, Hole cross, Elevated plus maze, Rearing, GroomingAbstract
Background: Mustard honey, a monofloral honey derived from mustard flower is considered a great source of nutritional and medicinal values. The honey is traditionally used as ethnomedicine in different parts of the world to cure many health problems. The present study aimed to evaluate its sedative-anxiolytic potential by integrating three conventional methods in a sequential order.
Methods: Open field, hole cross and elevated plus maze experiments were performed in a row with a single oral administration of honey to the Swiss Albino mice. Behavioral parameters like square crossing, rearing, grooming, hole crossing and entry/duration in open arm were observed for each animal in different time intervals.
Results: The findings were compared to that of a standard drug, diazepam (1 mg/kg). Mustard honey at higher doses showed sedative activity (4 g/kg and 6 g/kg) whereas with low doses (2 g/kg) exhibited anxiolytic potential. The physicochemical properties of honey were also screened in this study.
Conclusions: The integrated method proved to be an effective approach for assessment of neuropharmacological potential for crude or standard medicine. However, further analysis was recommended to investigate active compound which may lead to a new drug development.Metrics
References
Minnesota Wildflowers. Brassica nigra (Black Mustard), 2013. Available at: https://www.minnesot awildflowers.info/flower/black-mustard. Accessed on 15 May 2019.
Khalil MI, Motallib MB, Anisuzzaman ASM, Sathi ZS, Hye MA, Shahjahan M. Antibacterial Activities of Different Brands of Unifloral Honey Available at the Northern Region of Bangladesh. J Med Sci. 2001;1(6):389-92.
Linkon MR, Prodhan UK, Elahi T, Talukdar J, Alim MA. Comparative Analysis of the Physico-chemical and Antioxidant Properties of Honey Available in Tangail, Bangladesh. Universal J Food Nutr Sci. 2015;3(1):19-22.
Busto U, Sellers EM. Pharmacologic aspects of benzodiazepine tolerance and dependence. J Subst Abuse Treat. 1991;8(1-2):29-33.
Vinkers CH, Olivier B. Mechanisms Underlying Tolerance after Long-Term Benzodiazepine Use: A Future for Subtype-Selective GABA(A) Receptor Modulators. Adv Pharmacol Sci. 2012;416864.
Longo LP, Johnson B. Addiction: Part I. Benzodiazepines—Side Effects, Abuse Risk and Alternatives. Am Fam Physician. 2000;61(7):2121-8.
Bogdanov S. Physical properties of honey. In: Book of Honey. Chapter 4. Bee Product Science. Available at: www.bee-hexagon.net. Accessed on 3 January 2019.
International Standard Organisation. “Fruit and Vegetable Products—Determination of Soluble Solids—Refractometric Method”. 2nd Edition. International Organisation for Standardisation, Geneva; 1998.
Iftikhar F, Mahmood R, Islam N, Sarwar G, Masood MA, Shafiq H. Physicochemical Analysis of Honey Samples Collected from Local Markets of Rawalpindi and Islamabad, Pakistan. American Journal of Biochemistry. 2014;4(2):35-40.
Wakhle DM. Beekeeping technology – production, characteristics and uses of honey and other products. In: Mishra RC (ed). Perspectives in Indian Apiculture, Agro-Botanica, Bikaner; 1997: 134–139.
United States Standards for Grades of Extracted Honey. United State Department of Agriculture, Washington DC; 1985.
Kinoo MS, Mahomoodally MF, Puchooa D. Anti-Microbial and Physico-Chemical Properties of Processed and Raw Honeys in Mauritius. Advances in Infectious Diseases, 2012;2:25-36.
Billah MM, Rayhan MA, Yousuf SA, Nawrin K, Rayhan J, Khengari EM. A Novel Integrated (OF-HC-EPM) Approach to Study Anxiety Related Depressive Behavior in Mice Model: A Comparison of Neuro Standards. Adv Pharmacol Pharm. 2019;7(3):39-48.
Habib MR, Billah MM, Nawrin K, Hasan MR, Khalil MI, Rahman MM. Assessment of Analgesic and Neuropharmacological Activity of Different Extracts of Euphorbia hirta (Linn.) Leaf. Br J Pharm Res. 2016;9(2):1-7.
Pardon MC, Gould GG, Garcia A, Phillips L, Cook MC, Miller SA, et al. Stress reactivity of the brain noradrenergic system in three rat strains diff ering in their neuroendocrine and behavioral responses to stress: implications for susceptibility to stress-related neuropsychiatric disorders. Neuroscience. 2002;115(1):229–42.
Billah MM, Hasan MR, Nawrin K, Mohiuddin, Habib MR. Evaluation of Analgesic and Sedative-anxiolytic Potential of Paderia foetida Leaf Extract. Am J Biomed Sci. 2015;7(2):98-104.
Takagi K, Watanabe M, Saito H. Studies on the spontaneous movement of animals by the hole cross test: Effect of 2-dimethylaminoethane. Its acylates on the central nervous system. Jpn J Pharmacol. 1971;21(6):797-810.
Nawrin K, Billah MM, Jabed MSU, Roy A, Ahmad AKMR, Islam MN. Antipyretic, Antidiabetic, Thrombolytic and CNS Depressant Potential of Ethanol Extract of Crotalaria Verrucosa L. Leaves. Am J Biomed Sci. 2015;7(4):198-204.
Verma P, Hellemans KGC, Choi FY, Yu W, Weinberg J. Circadian phase and sex effects on depressive/anxiety-like behaviors and HPA axis responses to acute stress. Physiol Behav. 2009;99:276–85.
Hawiset T, Muchimapura S, Wattanathorn J, Sripanidkulchai B. Screening neuropharmacological activities of Kaempferia parviflora (Krachai dam) in healthy adult male rats. Am J Appl Sci. 2011;8(7):695-702.
Sestakova N, Puzserova A, Kluknavsky M, Bernatova I. Determination of motor activity and anxiety-related behaviour in rodents: methodological aspects and role of nitric oxide. Interdiscip Toxicol. 2013;6(3):126–35.
Pellow S, Chopin P, File SE, Briley M. Validation of open : closed arm entries in an elevated plus-maze as a measure of anxiety in the rat. J Neurosci Methods. 1985;14:149–67.
Walsh RN, Cummim RA. The open-field test - a critical review. Psychol Bull 1976;83(3):482-504.
Rodgers RJ, Dalvi A. Anxiety, defence and the elevated plus-maze. Neurosci Biobehav Rev. 1997;21:801-10.
Nicholas EC, James CB. Classics in Chemical Neuroscience: Diazepam (Valium), ACS. Chem Neurosci. 2014;5(4):253−60.
Zakusov VV, Ostrovskaya RU, Kozhechkin SN, Markovich VV, Molodavkin GM, Voronina TA. Further evidence for GABA-ergic mechanisms in the action of benzodiazepines. Arch Int Pharmacodyn Ther.1977;229(2):313–26.
Liebsch G, Montkowski A, Holsboer F, Landgraf R. Behavioural profiles of two Wistar rat lines selectively bred for high or low anxiety-related behaviour. Behav Brain Res. 1998;94(2):301–10.