DOI: http://dx.doi.org/10.18203/issn.2454-2156.IntJSciRep20205035

Recommended parameters for hepatocyte isolation-a review

Sushrutha Chikkanayakanahalli S., Sandhya Kalappa, Savitha Karlwad, Elango E. Murugaian

Abstract


There is an increasing demand for primary human hepatocytes for toxico-pharmacological studies and for exploring their role in liver cell transplantation. During the whole process of isolation, culture and cryopreservation hepatocytes are subjected to various stress. All these factors will lead to the impairment in the metabolic capability of the hepatocytes post isolation. Hepatocyte isolation is an expensive and resource driven procedure. Thus, it is important to consider all the factors before we subject the specimen for isolation of cells. By considering the factors that influence hepatocyte isolation, hepatocytes of good quality can be obtained. With recommendations on the ideal parameters for isolation being made the researcher shall have a knowledge on what critical factors need to be taken care of during hepatocyte isolation. There will be an opportunity to modify critical factors if possible, during the procurement of specimen by working in tandem with the clinicians. The specimen which shall be deemed un fit when two or more factors have exceeded the critical limits can be rejected.


Keywords


Hepatocytes, Culture, Isolation

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References


Terry C, Mitry RR, Lehec SC, Muiesan P, Rela M, Heaton ND et al. The effects of cryopreservation on human hepatocytes obtained from different sources of liver tissue. Cell Transplant. 2005;14(8):585-94.

Horner R, Kluge M, Gassner J, Nösser M, Major RD, Reutzel-Selke A. Hepatocyte Isolation After Laparoscopic Liver Resection. Tissue Eng Part C Methods. 2016 Sep;22(9):839-46.

Terry C, Hughes RD. An optimised method for cryopreservation of human hepatocytes. Methods Mol Biol. 2009;481:25-34.

Alexandre E, Cahn M, Abadie-Viollon C, Meyer N, Heyd B, Mantion G et al. Influence of pre-, intra- and post-operative parameters of donor liver on the outcome of isolated human hepatocytes. Cell Tissue Bank. 2002;3(4):223-33.

Hughes RD, Mitry RR, Dhawan A, Lehec SC, Girlanda R, Rela M, et al. Isolation of Hepatocytes from Livers from Non- Heart-Beating Donors for Cell Transplantation. liver transplantation. 2006;12:713-17.

Donato MT, Lahoz A, Montero S, Bonora A, Pareja E, Mir J et al. Functional assessment of the quality of human hepatocyte preparations for cell transplantation. Cell Transplant. 2008;17(10-11):1211-9.

Bonora-Centelles A1, Donato MT, Lahoz A, Pareja E, Mir J, Castell JV et al. Functional characterization of hepatocytes for cell transplantation: customized cell preparation for each receptor. Cell Transplant. 2010;19(1):21-8.

Matsumura M, Imura T, Inagaki A, Ogasawara H, Fukuoka K, Fathi I et al. A Simple and Useful Predictive Assay for Evaluating the Quality of Isolated Hepatocytes for Hepatocyte Transplantation. Scientific reports. 2019;9:6166.

Habibullah CM. Hepatocyte transplantation: need for liver cell bank. Trop Gastroenterol. 1992;13(4):129-32.

Lee CA, Dhawan A, Iansante V, Lehec S, Khorsandi SE, Filippi C. Cryopreserved Neonatal Hepatocytes May Be a Source for Transplantation: Evaluation of Functionality Toward Clinical Use. Liver transplantation. 2018;24(3):394-406.

Wolfgang Rudolf Vondran F, Katenz E, Schwartlander R, Morgul MH, Raschzok N, Gong X, et al. Isolation of Primary Human Hepatocytes After Partial Hepatectomy: Criteria for Identification of the Most Promising Liver Specimen. Artificial Organs. 2007:32(3):205-13.

Miller MA. Gender-based differences in the toxicity of pharmaceuticals-the Food and Drug Administration’s perspective. Int J Toxicol. 2001;20:149-52.

Mennecozzi M, Landesmann B, Palosaari T, Harris G, Whelan M. Sex Differences in Liver Toxicity—Do Female and Male Human Primary Hepatocytes React Differently to Toxicants In Vitro? PLoS ONE. 2015;10(4):e0122786.

Sharma A, Houshyar R, Bhosale P, Choi J-II, Gulati R, Lall C. Chemotherapy induced liver abnormalities: an imaging perspective. Clin Mol Hepatology. 2014;20(3):317-26.

Baron F, Beguin Y. Hepatic complications of chemotherapy. From trivial cytolysis to hepatic veno-occlusive disease. Rev Med Liege. 1997;52(2):93-7.

Meng FY, Liu L, Liu J, Li C-Y, Wang J-P, Yang F-H et al. Hepatocyte isolation from resected benign tissues: Results of a 5-year experience. World J Gastroenterol. 2016;22(36):8178-86.

Noor Mohamed MS, Kanwar A, Ray C, Wright MC, Cowie DE, Stamp S et al. Extracorporeal membrane oxygenation for resuscitation of deceased cardiac donor livers for hepatocyte isolation. J Surg Res. 2013;183(2).

Baccarani U, Sanna A, Cariani A, Sainz-Barriga M, Adani GL, Zambito AM. Isolation of Human Hepatocytes From Livers Rejected for Liver Transplantation on a National Basis: Results of a 2-Year Experience. Liver Transplantation. 2003;9(5):506-12.

Lee SM, Schelcher C, Demmel M, Hauner M, Thasler WE. Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure. J Vis Exp. 2013;79:e50615.

Green CJ, Charlton CA, Wang L-M, Silva M, Morten KJ, Hodson L. The isolation of primary hepatocytes from human tissue: optimising the use of small non-encapsulated liver resection surplus. Cell Tissue Bank. 2017;18:597-604.

Alexandre E, Cahn M, Abadie-Viollon C, Meyer N, Heyd B, Mantion G et al. Influence of pre-, intra- and post-operative parameters of donor liver on the outcome of isolated human hepatocytes. Cell Tissue Bank. 2002;3(4):223-33.

Lloyd TD, Orr S, Patel R, Crees G, Chavda S, Vadyar H, et al. Effect of patient, operative and isolation factors on subsequent yield and viability of human hepatocytes for research use. Cell Tissue Bank. 2004;5:81-7.

Fisher RL, Gandolfi AJ, Brendel K. Human liver quality is a dominant factor in the outcome of in vitro studies. Cell Biol Toxicol. 2001;17:179-89.

Izamis M-L, Perk S, Calhoun C, Uygun K, Yarmush ML, Berthiaume F. Machine Perfusion Enhances Hepatocyte Isolation Yields from Ischemic Livers. Cryobiol. 2015;71(2):244-55.

Duseja A, Najmy S, Sachdev S, Pal A, Sharma RR, Marwah N et al. High prevalence of non-alcoholic fatty liver disease among healthy male blood donors of urban India. J Gastroenterol Hepatol. 2019;(3):133-9.

Kalra S, Vithalani M, Gulati G, Kulkarni CM, Kadam Y, Pallivathukkal J et al. Study of prevalence of nonalcoholic fatty liver disease (NAFLD) in type 2 diabetes patients in India (SPRINT). J Asso Physicians India. 2013;61(7):448-53.

Baccarani U, Donini A, Risaliti A, Piccolo G, Dialti V, Cautero N et al. Steatotic versus cirrhotic livers as a source for human hepatocyte isolation. Transplant Proc. 2001;33:664-5.

Woolbrighta BL, Dorkoa K, Antoineb DJ, Clarkeb J, Gholamic P, Lia F et al. Bile acid-induced necrosis in primary human hepatocytes and in patients with obstructive cholestasis. Toxicol Applied Pharmacol. 2015;283(3):168-77.

Werner M, Driftmann S, Kleinehr K, Kaiser GM, Mathé Z, Treckmann J-W et al. All-In- One: Advanced preparation of Human Parenchymal and Non-Parenchymal Liver Cells. PLoS ONE. 2015;10(9):e0138655.

Aghdaie MH, Geramizadeh B, Azarpira N, Esfandiari E, Darai M, Rahsaz M, et al. Hepatocyte Isolation From Unused/Rejected Livers for Transplantation: Initial Step Toward Hepatocyte Transplantation, the First Experience From Iran. Hepat Mon. 2013;13(8):e10397.

Bachmann A, Moll M, Gottwald E, Nies C, Zantl R, Wagner H. 3D Cultivation Techniques for Primary Human Hepatocytes. Microarrays. 2015;4:64-83.