Acute effect of cyclophosphamide on rat’s urinary bladder and the possible protective role of sulforaphane: a histological and ultrastructural study


  • Rasha A. Elsisy Department of Anatomy and Embryology, Faculty of Medicine, Kafrelsheikh University, Egypt
  • Medhat F. Taha Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Egypt Anatomy Department, Al- Qunfudah Medical College, University of Umm AL-Qura, Makka, Saudi Arabia
  • Hany M. A. Sonpol Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Egypt Basic medical sciences, College of Medicine, University of Bisha, Saudi Arabia
  • Turki A. S. Baokbah Department of Medical Emergency Service, Al- Qunfudah Health Sciences College, University of Umm AL-Qura, Makka, Saudi Arabia
  • Mona A. Abdelkareem Department of Anatomy and Embryology, Faculty of Medicine, Kafrelsheikh University, Egypt
  • Amira E. Farage Department of Anatomy and Embryology, Faculty of Medicine, Kafrelsheikh University, Egypt



Cyclophosphamides, Sulforaphane, Antioxidants and cystitis


Background: Cyclophosphamide disturbs the oxidant and antioxidant balance that is associated with several unwanted toxic effects and induction of secondary cancers. The aim of this study was to test the protective effects of Sulforaphane on the cyclophosphamide toxicity of rat urinary bladder.

Methods: 32 male albino rats were divided into 4 groups, 8 animal each (n=8). Group I received saline intra-peritoneal, group II received 5 mg/kg sulforaphane for 5 days and then saline, group III received 0.9% saline intra-peritoneal for consecutive 5 days and a single dose of cyclophosphamide 200 mg/kg on the six-day, group IV received sulforaphane at a dose of 5 mg/kg for consecutive 5 days and a single dose of cyclophosphamide 200 mg/kg on the six day. On the seventh day of the experiment, the animals were sacrificed, and the urinary bladder samples were dissected for histopathological and immunohistochemical investigations and electron microscopic studies.

Results: the mucosa of the urinary bladder of Sulforaphane treated group showed normal architecture while that of cyclophosphamides treated group showed features of degenerated and ulcerated lesions of the epithelial lining associated with hemorrhage. Theses lesions markedly decreased in the mucosa of urinary bladder of cyclophosphamides and sulforaphane treated group.

Conclusions: The use of sulforaphane reduces the cyclophosphamide toxicity on the urinary bladder in the form of decreased vacuolation with decreased degeneration of the epithelial lining.


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Author Biography

Medhat F. Taha, Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Egypt Anatomy Department, Al- Qunfudah Medical College, University of Umm AL-Qura, Makka, Saudi Arabia

Anatomy and Embryology department


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